Fig. 3. (A) Distribution of DNA damage from metals exposed cells treated with gamma-radiation (3Gy),1 and 24 h post-exposure, analyzed by neutral comet assay (B) DNA DSBs in irradiated Balb/c 3T3 cells previously exposed to metal mixture at 5 min (3 Gy), 1 h and 24 h post-exposure and evaluated with the neutral comet assay protocol. Results are presented in terms of Tail length (microns); dose of gamma radiation=3 Gy, n=2 slides/condition, 50 comets/slide, mean±s.e., two-tailed unpaired t-test, *p<0.05. (C) A representative neutral comet images (20X).